Asian Journal of Pharmaceutics
  Home  |  About us  |  Instructions  |  Search  |  Current issue  |  Archives  |  Submission  |  Subscribe  |  Contact  |  Login 

Table of Contents   
Year : 2010  |  Volume : 4  |  Issue : 4  |  Page : 173-178
Probiotic-assisted colon-specific delivery of diclofenac sodium from guar gum matrix tablets: In vitro evaluation

1 Keegad Biogen Pvt Ltd, Naraina Industrial Area Phase II, New Delhi, India
2 TIFAC-Core in Green Pharmacy, Pharmaceutical Research Division, B R Nahata College of Pharmacy, Mandsaur, MP, India

Click here for correspondence address and email

Date of Web Publication15-Feb-2011


Purpose : The aim of present work was to investigate the in vitro release profile of diclofenac sodium from matrix tablets, which were prepared using guar gum with and without probiotics. Methods : Six matrix tablets formulations (F1-F6) of diclofenac sodium containing varying proportions of guar gum, 10-60% of tablet weight were prepared by wet granulation method and evaluated in vitro for their release profile. Four matrix tablets formulations (F7-F10) of diclofenac with guar gum (40%) were prepared incorporating varying concentration of commercial spores of lactobacillus and bifidobacterium species that are commonly found in colon microflora. Results : The tablets having same concentration of guar gum without probiotics (F4) showed a 54.47% cumulative release of diclofenac after 24 hr while that in presence of probiotics (F9) was 73.01%. These drug release studies were conducted without rat caecal content. In another set of experiments, the drug release studies were carried out in presence of rat caecal content. In such experiments, more than 95% drug was released from F4 and F9 formulations after 24 hr. Conclusion : The results showed that commercial probiotics are capable in digesting guar gum. These probiotics may be used to assist in colon delivery of drugs from formulations prepared with guar gum.

Keywords: Colon, diclofenac, guar gum, matrix tablet, probiotics

How to cite this article:
Ghosh PK, Gupta VB, Gondoliya B, Rathore MS. Probiotic-assisted colon-specific delivery of diclofenac sodium from guar gum matrix tablets: In vitro evaluation. Asian J Pharm 2010;4:173-8

How to cite this URL:
Ghosh PK, Gupta VB, Gondoliya B, Rathore MS. Probiotic-assisted colon-specific delivery of diclofenac sodium from guar gum matrix tablets: In vitro evaluation. Asian J Pharm [serial online] 2010 [cited 2015 Aug 30];4:173-8. Available from:

   Introduction Top

Site-specific drug delivery is preferred in order to reduce harmful adverse effects and enhance pharmacological response concerned with the drug. Colon is considered as a potential site to deliver therapeutic agents in clinical conditions such as colon cancer, irritable bowel syndrome, ulcerative colitis and Crohn's disease. [1],[2] Colon could be viewed as desirable site for oral delivery of protein and peptide drugs due to low diversity and intensity of digestive enzymes and near neutral pH. Long retention time of dosage form in colon offers suitability of prolong drug delivery in certain clinical conditions that have peak symptoms in the early morning that exhibit circadian rhythms like nocturnal asthma, rheumatoid arthritis, ulcer disease and ischemic heart diseases. [3],[4],[5] Rectal route of drug delivery to the distal gut is inconvenient and patient noncompliant. [6] Henceforth, oral route is a preferred route for colonic drug delivery.

Colon-specific drug delivery via oral route faces several challenges. The drug must neither be absorbed from upper GIT nor be degraded in small intestine lumen. To serve the purpose-desired dosage form prevents drug release in the upper GIT and deliver maximum drug content in the colon. Formation of prodrug, multicoating time-dependent delivery, coating with pH-sensitive polymers, pressure-dependent delivery and to system desired making use of biodegradable polymers are certain strategies currently being approached for colon-specific drug delivery. [7],[8],[9]

Biodegradable polymer (viz polysaccharides)-based drug delivery approach seems quite promising. Polysaccharides arriving from small intestine are the main source of nourishment for microflora in the colon. The ability of colon microflora to degrade polysaccharides such as pectin, guar gum, chitosan, etc. forms the basis of formulation development for colon-specific drug delivery. [10],[11] The colonic microflora must be present in sufficient number to digest the carrier (like guar gum) to ensure the release of the drug at colon. [12] However, intersubject variation of colonic microflora, sterilization of colonic microflora by antibiotics (commonly prescribed to treat colonic disease) and slow enzymatic degradation are some limitations of this approach for colon-specific drug delivery. [13],[14] The limitations can be resolved by incorporating bacteria in spore form (probiotics) in formulation to assure successful and efficient drug release selectively to colon. Probiotic supplements (Bifidobacteria spp and Lactobacilli) are known to improve resistance to gut infections by inhibiting the growth of harmful bacteria, to reduce cholesterol levels, improve the immune response and produce vitamins. [15] Various probiotic-containing preparations like Sporlac (Lactobacillus) and Prowell (Bifidobacterium) are available in the market in spore form. Protective effect of probiotics (lactic acid producing bacteria) in colon carcinogenesis has also been demonstrated in clinical trials. [16] Incorporation of probiotics would provide the health benefits on one hand, while ensure drug release at colon in any condition on the other.

Patients who regularly take anti-inflammatory drug NSAID, have a 40-50% reduction in mortality from colorectal cancer. [17],[18] Cyclooxygenase-2 (COX-2) is upregulated in colorectal and other cancers. It is suggested that prostaglandins may act as tumor promoters and that inhibition of COXs may be chemoprotective. [19] Recentely diclofenac has been identified as a possible potential agent has chemopreventive effect particularly in colorectal cancer. [20],[21] Morphine-sparing effect of diclofenac in cancer pain is reported. [22] Like most of the NSAIDs, diclofenac is also associated with gastrointestinal problems. For such conditions, site-specific drug delivery of diclofenac to the colon is always desirable. Keeping the above in view, attempts were made to evaluate the in vitro drug delivery of diclofenac to the colon by incorporating the probiotics (lactobacillus and bifidobacterium spores) in guar gum matrix tablet.

   Materials and Methods Top

Diclofenac sodium (95-105% purity) was a gift sample from M/s. Torrent Laboratories, Ahmedabad, India. Guar gum was obtained from SD Fine Chemicals, Mumbai. Other materials used in the study such as lactose, talc, magnesium stearate were of analytical Grade. Sporlac; , Prowell; , Purchased from local medical store in Mandsuar. Double beam UV-visible Spectrophotometer (Thermospectronic, Merck, Mumbai), Brookfield viscometer (RVT/282889, Brookfield, USA) were used for analysis of samples.

In vitro digestion studies of guar gum by bacterial spores

In 200 ml guar gum (1% w/v) slurry one sachet of Sporlac (1 gm) and one capsule of Prowell (525 mg) was dispersed separately (test 1 and test 2, [Table 3]) and incubated at 37ºC in incubator. At different time interval the change in pH and viscosity was measured using calibrated pH meter (Remi, Mumbai) and Brookfield viscometer, respectively.

Formulation of matrix tablets of diclofenac with guar gum

Matrix tablets of diclofenac sodium (75 mg) using analytical grade guar gum were prepared by wet granulation method. Lactose was used as diluent and the mixture of talc and magnesium stearate at 2:1 ratio was used as lubricant. Guar gum was sieved (sieve no. 60) separately and mixed with Diclofenac Sodium (sieve no. 100) and lactose (sieve no. 60). The composition of different formulations is shown in [Table 1]. The powders were blended and granulated with water. The wet mass obtained was then passed through sieve no. 14 and wet granules were dried at 50ºC for 2 h. The dried granules were passed through a sieve no. 16 and were lubricated with a mixture of talc and magnesium stearate (2:1). The lubricated granules were compressed at compression force 5000-6000 kg using 12-mm-round concave-convex punch on eight-station rotary tablet machine (M/s Kambert Machinery Co. Pvt. Ltd., Ahmedabad, India).
Table 1: Formulation of diclofenac sodium tablets* prepared with varying amount of guar gum and probiotics (sporlac and prowell)

Click here to view

Formulation of matrix tablet of diclofenac with guar gum containing probiotics

Matrix tablets of diclofenac sodium were prepared using guar gum (40% of tablet weight) by the wet granulation method using water as granulating agent.

Sporlac (containing Lactobacillus sporogens) and Prowell (containing Lactobacillus acidophilus, Bifidobacterium bifidum, Bifidobacterium longum, Bifidobacterium infantis spores) were used as probiotics. The method of preparation of tablets was same as described above with few modifications. Probiotics were added at two stages; half quantity was added before granulation and half quantity was added after granulation and the wet granules were dried at 40ºC for 2 hr. Compositions of different formulations is shown in [Table 1].

Granules of each composition after lubrication was compressed and tested for their hardness, drug content, and drug release characteristics with the required number of tablets for each test. The hardness of the matrix tablets was determined using a Monsanto hardness tester (M/s Campbell Electronics, Mumbai, India).

Determination of drug content

Ten tablets were finely powdered. Quantity of the powder equivalent to 100 mg of diclofenac sodium was accurately weighed and transferred to 100 ml volumetric flask containing 50 ml of 0.1 M NaOH. It was allowed to stand for 6 hr with intermittent sonication to ensure complete solubility of the drug. Then volume made up to 100 ml with 0.1 M NaOH and centrifuged at 500 rpm for 10 min. One milliliter of the supernatant liquid was suitably diluted, filtered and analyzed for diclofenac sodium content at 276 nm by double UV-visible spectrophotometer. [23]

In vitro dissolution studies

In vitro dissolution studies for all matrix tablet formulations were performed by using USP dissolution test apparatus (Apparatus 1, Basket type, 37ºC) at 100 rpm for 2 hr in 0.1 N HCl (900 ml).Then the dissolution medium was replaced with pH 7.4 phosphate buffer (900 ml) and tested for 3 hr as the average transit time of small intestine is 3 hr. After 5 hr, the dissolution medium was replaced with pH 6.8 phosphate buffer and tested for next 19 hr. At different time intervals a 10 ml of the sample was taken and analyzed for diclofenac sodium content at 276 nm by double beam UV-visible spectrophotometer. A 10 ml fresh dissolution medium was added to make the volume after each sample withdrawal. [23]

In vitro dissolution studies on guar gum diclofenac matrix tablet in presence of 4%w/v rat caecal content

To assess the susceptibility of guar gum being acted upon by the colonic bacteria, drug release studies were carried out in the presence of rat caecal content as described by Krishanaiya. [23] Appropriate approval from Institutional Animal Ethics Committee (Reg No. 918/ac/05/CPCSEA) was obtained (no.113/MPh/09) and animal experimentation was performed accordingly. In order to induce enzymes specifically acting on guar gum in the caecum, male Albino rats weighing 150-200 g and maintained on normal diet were intubated with teflon tubing and 1 ml of 2% wt/vol dispersion of guar gum in water was administered directly into the stomach. The tubing was removed and this treatment was continued for 3 days in one set of rats and for 7 days in another set of rats. Thirty minutes before the commencement of drug release studies, four rats were killed by spinal traction. The abdomen were opened, the caecai were isolated, ligated at both ends, cut loose and immediately transferred into pH 6.8 PBS, previously bubbled with carbon dioxide gas (CO 2 ). The caecal bags were opened, their contents were individually weighed, pooled and then suspended in PBS to give a final caecal dilution of 4% wt/vol. As the caecum is naturally anerobic, all these operations were carried out under CO 2 . The drug release studies were carried out in USP dissolution test apparatus (Apparatus 1, 100 rpm, 37ºC) with slight modification. A beaker (capacity 150 ml) containing 100 ml of dissolution medium was immersed in the water contained in the 1000 ml vessel, which in turn, was the water bath of the apparatus. The swollen formulations after completing the dissolution study in 0.1 M HCl (2 hr) and pH-7.4 phosphate buffer (3 hr) were placed in the baskets of the apparatus and immersed in the dissolution medium containing rat caecal content medium. The drug release studies were carried out up to 24 hr and 1 ml samples were withdrawn at specified time intervals without a pre-filter and replaced with 1 ml of fresh Sorenson's phosphate buffer bubbled with CO 2 . The volume was made upto 10 ml with Sorenson's phosphate buffer and centrifuged. The supernatant was filtered and filtrate was analyzed for diclofenac sodium content at 276 nm by double beam UV-Visible spectrophotometer.

   Results and Discussion Top

In vitro depolymerization studies on guar gum slurry in presence of commercial probiotics

L. acidophilus (Sporlac), B. bifidum, B. longum, B. infantis (prowell) are commonly prescribed as probiotics to balance disturbed intestinal microflora and related dysfunction of the gastrointestinal tract (GIT). In vitro digestion studies of guar gum slurry in presence of these bacterial species were performed to investigate the degradation effect of the bacteria on guar gum. In human intestine guar gum (a polysaccharide) is known to degrade by fermentation to short-chain fatty acids (SCFA) with evolution of CO 2 . [24],[25] The viscosity of the guar gum polysacchrides is likely to be reduced by deploymerization process with reduction in surrounding pH due to generation of SCFA and CO 2 . These two parameters were taken as indicator to investigate the depolymerization of guar gum in vitro in presence of the sporlac spores 1 g (test 1) and prowell 525 mg (test 2) and the results are shown in [Table 2]. Plain guar gum slurry (1% wt/vol) was kept as control to compare the changes in the viscosity. Initial pH and viscosity of test 1 was 6.98 and 2600 centipoises (cps), respectively while with test 2 it was 6.96 and 2600 cps. Up to 7 hr after incubation no change occurred in pH and viscosity of slurry. Decrease in viscosity and pH started after this point [Table 2] and at the end of 24 hr marked difference in pH and viscosity was observed in test 1 (pH 5.45, viscosity 600 cps) and test 2 (pH5.22 and viscosity 860 cps). pH and viscosity of control (guar gum only) remained unchanged during the period of incubation. The bacterial species used was in the spore form and first 7 hr required to become in vegetative form. This might be the reason for unchanged pH and viscosity in first 7 hr. The results suggest that the commercial probiotics sporlac and prowell are capable to degrade guar gum. Henceforth, diclofenac guar gum tablets containing probiotics may also be expected to release the drug during in vitro dissolution studies on digestion of guar gum by probiotics. With this objective, the tablets of guar gum in presence of probiotics were formulated. For comparison purpose plain guar gum tablets of diclofenac (without probiotic) were also formulated [Table 1].
Table 2: Changes in viscosity and pH of guar gum slurry in distilled water (1%wt/vol) on incubation with spores (sporlac and prowell) at 37°±2° C (Values are mean ± SEM, n = 3)

Click here to view

The hardness of all tablets was found between 5.5 and 6.5 kg/cm 2 . All formulation satisfied the drug content of diclofenac sodium and found between 98±2%.

In vitro drug release studies

Diclofenac and guar gum matrix tablets were prepared with and without probiotics with different proportions of guar gum and dissolution studies were performed according to a method described elsewhere. Diclofenac matrix tablet formulation code F1, F2, F3, F4, F5 and F6 were prepared with 10, 20, 30, 40, 50 and 60% guar gum wt/wt while diclofenac matrix tablet with probiotics sporlac and prowell were formulated with 40% guar gum (F7-F10). Formulation code F7, F8, F9 and F10 contained varying proportions of probiotics [Table 1]. All tablets retained their shape during the course of dissolution. However, the tablets were swelled and showed different cumulative percent release of diclofenac sodium as shown in [Table 3]. Diclofenac matrix tablets when come in contact with water the guar gum present in it swells and form a gel layer around the tablet. The drug then diffuse out from it and rate of diffusion depends on the amount of guar gum present in the tablet. It was observed that the tablets having lower amount of guar gum (F1;10% and F2; 20%) released diclofenac faster as compared to the higher guar gum-containing tablets F3, F4, F5 and F6. The cumulative percent release with F1 tablets was 21.1% in first 5 hr while 87.01% at the end of 24 hour was observed while that of with F4 was 12.37% and 54.47%, respectively. Formulation F4 contained 40% guar gum while F1 contained 10% guar gum. The higher concentration of guar gum retarded the release of the diclofenac sodium in first 5 hr up to 12.37% as compared to 21.1% from F1. Further increase in guar gum amount (F5 and F6) did not make remarkable difference in diclofenac release than from F4. The ideal colon-targeted oral drug delivery system is expected to release the minimal amount of drug in upper GIT i.e, stomach and small intestine while should release maximum amount of drug in colon. The first 5-hr drug release is considered analog to in vivo drug release in upper GIT. F4 tablets showed only 12.37% drug release in first 5 hr and are expected to release maximum drug to the colon provided the bacteria responsible to digest guar gum are present in sufficient number.
Table 3: In vitro drug release profile of colon-targeted matrix tablet formulation of diclofenac sodium prepared with guar gum 10% (F1), 20% (F2), 30% (F3), 40% (F4), 50% (F5) and 60% (F6) of tablet weight (values are mean ± SEM, n=3)

Click here to view

The diclofenac release was studied next from the tablets containing probiotics and 40% guar gum. Results are shown in [Table 4] and [Figure 1]. Cumulative percentage of drug release from F7 was 11.06% in first 5 hr while that was 63.96% at 24 hr. F7 tablets contained sporlac and prowell and the spores are expected to be become vegetative after 7 hr as revealed by guar gum depolymerization study and capable of degrading to guar gum, consequently the higher drug release at colon. The addition of sporlac and prowell did not affected the release of diclofenac in first 5 hr while enhanced the cumulative percent release at 24 hr. The situation mimics the condition as if colon microflora is present as happens in vivo at colon. As the amount of sporlac and prowell increased in formulation the cumulative drug release at 24 hr also increased. F9 tablets showed a cumulative percentage release of 73.01% of diclofenac after 24 hr. F10 (sporlac 0.8 billion spores and prowell 12 million spores) showed maximum release of diclofenac in 24 hr (75.53%) as compared to 54.47% from F4 tablets. However, there was not significant change in diclofenac release on increasing the amount of sporlac and prowell in F10 (75.53%) as compared to F9 (73.01%). The presence of the bacterial spores (probiotics) caused at least more than 70% of drug release after 24 hr. This indicates that the guar gum was degraded by sporlac and prowell that released more amount of drug as compared to the formulation without probiotic (F4). The incorporation of probiotics guar gum matrix tablet ensured at least 70% drug release at colon while protecting the diclofenac release in upper GIT
Table 4: Result of in vitro drug release study of colon-targeted diclofenac-guar gum matrix tablet containing probiotics (values are mean ± SEM, n=3). F4 tablets are without probiotics while F7, F8, F9 and F10 were prepared with varying proportions of probiotics.

Click here to view

The in vitro release studies of diclofenac from selected formulation F4 and F9 were conducted in absence and in presence of rat caecal content. The observations are shown in [Figure 1],[Figure 2] and [Figure 3]. Formulation code F4 showed 95.26 and 54.47 cumulative percent release of diclofenac in presence and in absence of caecal content, respectively. Formulation F9 containing probiotic (lactobacillus and bifidobacterium spores) showed 98.13 and 73.01% diclofenac release in presence and absence of rat caecal content, respectively. There was about 20% more release of diclofenac in F9 (containing probiotic) as compared to F4 (without probiotic) in absence of rat caecal content. The situation mimics the conditions of GIT without bacterial flora as happens in antibiotic therapy. Formulation F9 is expected to show better drug release in colon as compared to F4 in absence of colonic microflora and ensures major portion of drug in formulation to be released at the colon. Both formulations F4 and F9 showed almost similar amount of drug released (95 and 98%) in presence of colonic flora (rat caecal content).
Figure 1: Cumulative percent of diclofenac sodium released from colon-targeted matrix tablet containing 40% of guar gum (F4) of total tablet weight without and with 4% wt/vol rat caecal content

Click here to view
Figure 2: Cumulative percentage of diclofenac sodium released from colon-targeted matrix tablet containing probiotics in absence of rat caecal content

Click here to view
Figure 3: Cumulative percentage of diclofenac sodium released from colon-targeted matrix tablet containing probiotics (F9) without and with 4% w/v RCC

Click here to view

   Conclusions Top

On the basis of above study it may be concluded that the formulation containing probiotics ensures the major portion of drug (more than 70%) to be released at colon even in absence of colonic microflora. However, in vivo studies are needed to confirm the results obtained in in vitro studies. In present research work in vitro characterization of probiotic-assisted colon delivery of diclofenac was studied. The guar gum matrix tablet formulation developed with probiotic holds tremendous potential to deliver a variety of drugs in colon diseases (viz anticancer drugs) specifically at colon and ensures maximum drug concentration at colon even in case of disturbed GIT microflora on one hand while reduce the dose and frequency of the drug and consequently lowers drug-associated side effects on other.

   Acknowledgement Top

Authors are thankful to TIFAC, Government of India, BR Nahata College of Pharmacy and KEE GAD Biogen Pvt Ltd, New Delhi for providing necessary financial support to carry out the present research work. Authors are also thankful to M/s. Torrent Laboratories, Ahmedabad, India for providing diclofenac sodium gift sample.

   References Top

1.Friend DR. New oral delivery systems for treatment of inflammatory bowel disease. Adv Drug Del Rev 2005;57:247-65.   Back to cited text no. 1
2. Sinha VR, Mittal BR, Bhutani KK, Kumria R. Colonic drug delivery of 5-fluorouracil: An in vitro evaluation. Int J Pharm 2004;269:101-8.   Back to cited text no. 2
3.Watts P, Illum L. Colonic drug delivery. Drug Dev Ind Pharm 1997;23:893-913.  Back to cited text no. 3
4.Fatima LAA, Chandran S. Multiparticulate formulation approach to colon specific drug delivery: Current perspectives. J Pharm Pharmaceut Sci 2006;3:327-38.  Back to cited text no. 4
5. Libo Y, James SC, Joseph AF. Colon-specific drug delivery: New approaches and in vitro/in vivo evaluation. Int J Pharm 2002;235:1-15.  Back to cited text no. 5
6.Vandamme TF, Lenourry A, Charrueau C, Chaumeil JC. The use of polysaccharides to target drugs to the colon. Carbohydrate Polymers 2002;48:219-31.  Back to cited text no. 6
7.Ishibashi T, Hatano H, Kobayashi M, Mizobe M, Yoshino H. In vivo drug release behaviour in dogs from a new colon-targeted delivery system. J Control Release 1999;57:45-53.  Back to cited text no. 7
8.Chourasia MK, Jain SK. Pharmaceutical approaches to colon targeted drug delivery systems. J Pharm Pharmaceut Sci 2003;6:33-66.  Back to cited text no. 8
9.Jain NK. Advances in controlled and novel drug delivery. 1 st ed. New Delhi: CBS Publishers and Distributors; 2001.  Back to cited text no. 9
10.Wilson PJ, Basit AW. Exploiting gastrointestinal bacteria to target drugs to the colon: An in vitro study using amylose coated tablets. Int J of Pharm 2005;300:89-94.   Back to cited text no. 10
11.Rubinstein A. Microbially controlled drug delivery to the colon. Bioph Drug Dispos 1990;11:465-75.  Back to cited text no. 11
12.Sinha VR, Mittal BR, Kumria R. In vivo evaluation of time and site of disintegration of polysaccharide tablet prepared for colon-specific drug delivery. Int J Pharm 2005;289:79-85.  Back to cited text no. 12
13.Rubinstein A, Radai R, Ezra M, Pathak S, Rokem S. In vitro evaluation of calcium pectinate: A potential colon-specific drug delivery carrier. Pharm Res 1993;10:258-63.  Back to cited text no. 13
14. Sinha VR, Kumria R. Polysaccharides in colon-specific drug delivery. Int J Pharm 2001;241:19-38.  Back to cited text no. 14
15.Tuohy KM, Kolid S, Lustenberger AM, Gibson GR. The prebiotic effects of biscuits containing partially hydrolysed guar gum and fructo-oligosaccharides - a human volunteer study. Br J Nutr 2001;86:341-8.   Back to cited text no. 15
16. Singh J, Rivenson A, Tomita M, Shimamura S, Ishibashi N, Reddy BS. Bifidobacterium longum, a lactic acid-producing intestinal bacterium inhibits colon cancer and modulates the intermediate biomarkers of colon carcinogenesis. Carcinogenesis 1997;18:833-41.   Back to cited text no. 16
17.Muscat JE, Stellman SD, Wynder EL. Nonsteroidal antiinflammatory drugs and colorectal cancer. Cancer (Phila) 1994;74:1847-54.  Back to cited text no. 17
18.Stürmer T, Buring J, Lee I, Kurth T, Gaziano J, Glynn R. Colorectal Cancer After Start of Nonsteroidal Anti-Inflammatory Drug Use. Am J Med 2006;119:494-502.  Back to cited text no. 18
19.Hixson LJ, Alberts DS, Krutzsch M, Einsphar J, Brendel K, Gross PH, et al. Antiproliferative effect of nonsteroidal anti-inflammatory drugs against human colon cancer cells. Cancer Epidemiol Biomarkers Prev 1994;3:433-8.  Back to cited text no. 19
20.Seed MP, Brown JR, Freemantle CN, Papworth JL, Colville-Nash PR, Willis D, et al. The inhibition of colon-26 adenocarcinoma development and angiogenesis by topical diclofenac in 2.5% hyaluronan. Cancer Res 1997;57:1625-9.  Back to cited text no. 20
21.Falkowski M, Skogstad S, Shahzidi S, Smedsröd B, Sveinbjörnsson B. The effect of cyclooxygenase inhibitor diclofenac on experimental murine colon carcinoma. Anticancer Res 2003;23:2303-8.  Back to cited text no. 21
22.Bjorkman R, Ullman A, Hedner J. Morphine-sparing effect of Diclofenac in cancer pain. Eur J Clin Pharmacol 1993;44:1-5.  Back to cited text no. 22
23.Krishnaiah YS, Satyanarayana S, Rama Prasad YV, Narasimha SR. Evaluation of guar gum as a compression coat for drug targeting to colon. Int J Pharm 1998;171:137-46.  Back to cited text no. 23
24.Tomlin J, Read NW, Edwards CA, Duerden BI. The degradation of guar gum by a faecal incubation system. Br J Nutr 1986;55:481-6.  Back to cited text no. 24
25.Tomlin J, Read NW. The relation between bacterial degradation of viscous polysaccharides and stool output in human beings. Br J Nutr l988;60:461-75.  Back to cited text no. 25

Correspondence Address:
Mahendra Singh Rathore
Pharmaceutical Research Division, Tifac-Core in Green Pharmacy, Mandsaur, MP-458 001
Login to access the Email id

Source of Support: None, Conflict of Interest: None

DOI: 10.4103/0973-8398.76734

Get Permissions


  [Figure 1], [Figure 2], [Figure 3]

  [Table 1], [Table 2], [Table 3], [Table 4]

This article has been cited by
1 Guar gum as platform for the oral controlled release of therapeutics
Tejraj M Aminabhavi,Mallikarjuna N Nadagouda,Shrinivas D Joshi,Uttam A More
Expert Opinion on Drug Delivery. 2014; : 1
2 Emerging techniques and challenges in colon drug delivery systems
Singh, G. and Kumar, D. and Singh, M. and Sharma, D. and Kaur, S.
Journal of Applied Pharmaceutical Science. 2012; 2(3): 139-147
3 Probiotic assisted colon targeted drug delivery system: Research scope
Pooja, C. and Naveen, M. and Rathore, M.S. and Anurekha, J. and Kumar, J.A.
Asian Journal of Pharmaceutical and Clinical Research. 2011; 4(SUPPL. 2): 12-15


    Similar in PUBMED
   Search Pubmed for
   Search in Google Scholar for
 Related articles
    Email Alert *
    Add to My List *
* Registration required (free)  

    Materials and Me...
    Results and Disc...
    Article Figures
    Article Tables

 Article Access Statistics
    PDF Downloaded20    
    Comments [Add]    
    Cited by others 3    

Recommend this journal