In Vivo Neuroprotective Activity of Erythropoietin-Alginate Microspheres at Different Polymer Concentrations

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Dewi Melani Hariyadi

Abstract

Aim: The aim of this study was to evaluate in vivo neuroprotectant activity of erythropoietin-alginate microspheres with different concentrations to modify release to reduce the administration frequency of erythropoietin. Materials and Methods: Sodium alginate and CaCl2 were used to produce microspheres using aerosolization technique and erythropoietin as model. Balb/c-strain mice (Mus musculus) were used to study in vivo activity in terms of locomotor activity and glutathione peroxidase (GSHPx) activity as well as percentage of reticulocytes. Results and Discussion: Erythropoietin-alginate microspheres demonstrated neuroprotectant activity such as locomotor activity and GSHPx activity compared to erythropoietin alone and blank microspheres. Increased polymer concentrations (1–3%), however, did not have a significant effect on changes of the effectiveness of the activity of microspheres. Interestingly, these results also showed that erythropoietin-alginate microspheres which contain only 5000 units produced a high percentage of reticulocytes. Conclusion: The study indicates that erythropoietin-alginate microspheres showed the potential activity of erythropoietin-alginate microspheres although in low concentration. Increased concentrations of polymers showed similar effects in locomotors activity and GSHPx enzyme activity as parameters of neuroprotectant activity.

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How to Cite
Hariyadi, D. M. (2018). In Vivo Neuroprotective Activity of Erythropoietin-Alginate Microspheres at Different Polymer Concentrations. Asian Journal of Pharmaceutics (AJP), 12(04). https://doi.org/10.22377/ajp.v12i04.2833
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ORIGINAL ARTICLES