@article{Kasimedu_2024, title={A Novel UPLC Method for the Estimation of Antidiabetic Drugs in Bulk and its Tablet dosage form}, volume={17}, url={http://www.asiapharmaceutics.info/index.php/ajp/article/view/5110}, DOI={10.22377/ajp.v17i04.5110}, abstractNote={<p>A highly sensitive, precise, and accurate ultra-performance liquid chromatography method was developed and<br>validated for the determination of ertugliflozin and sitagliptin in the combined formulation. Chromatographic<br>separation was carried out on the DIKMA Endeversil C18 column (2.1 mm × 50 mm, 1.7 μm) using the mobile<br>phase of KH2PO4:methanol 45:55 v/v. The common wavelength of absorption of ertugliflozin and sitagliptin was<br>found to be 225 nm. The flow rate was maintained at 0.3 mL/min, with 2 µL injection volume. The retention<br>time of ertugliflozin and sitagliptin was found to be 0.41 min and 0.535 min. % relative standard deviation of the<br>ertugliflozin and sitagliptin was found to be 0.681 and 0.218, respectively. %recovery was obtained as 100.146%<br>and 100.27% for ertugliflozin and sitagliptin, respectively. Limit of detection (LOD), limit of quantification<br>(LOQ) values obtained from regression equations of ertugliflozin and sitagliptin were 2.91, 2.96, and 10.04,<br>10.09, respectively. Regression equation of ertugliflozin is y = 1329.8 × −228.7 (0.9997) and y = 1294.4.× –40.1<br>of sitagliptin (0.9998). The proposed method was validated in terms of linearity, precision, accuracy, specificity,<br>LOD, LOQ, and robustness. The method was successfully applied to the estimation of ertugliflozin and sitagliptin<br>tablet dosage forms.</p>}, number={04}, journal={Asian Journal of Pharmaceutics (AJP)}, author={Kasimedu, Saravanakumar}, year={2024}, month={Jan.} }