TY - JOUR AU - Annapurna, Dr. Mukthinuthalapati Mathrusri PY - 2022/12/15 Y2 - 2024/03/28 TI - Simultaneous Estimation of Dolutegravir Sodium, Emtricitabine, and Tenofovir Disoproxil Fumarate by UPLC JF - Asian Journal of Pharmaceutics (AJP) JA - AJP VL - 16 IS - 4 SE - ORIGINAL ARTICLES DO - UR - http://www.asiapharmaceutics.info/index.php/ajp/article/view/4623 SP - AB - <p>Introduction: A new reversed-phase ultra-fast liquid chromatography (RP-UPLC) method has been developed<br>for the simultaneous quantification of Dolutegravir sodium, Emtricitabine, and Tenofovir disoproxil fumarate.<br>Dolutegravir, Emtricitabine, and Tenofovir disoproxil fumarate are anti-retroviral drugs. Dolutegravir sodium is<br>an human immunodeficiency virus integrase inhibitor. Emtricitabine and Tenofovir disoproxil fumarate are reverse<br>transcriptase enzyme inhibitors. Materials and Methods: Shimadzu NexeraX2 Model UPLC system with PDA<br>detector and Shim-pack C18 column was employed for the chromatographic study. Mobile phase consisting of<br>0.1% Tri ethyl amine (Adjusted to pH 6.0 with ortho phosphoric acid): Acetonitrile (55: 45) was used with 1.0 mL/<br>min flow rate and UV detection at 260 nm. Results and Discussion: Beer-Lambert’s law was obeyed over the<br>concentration range 5–400, 2–150 and 5–500 μg/mL with linear regression equation y = 1211.7× + 506.73 (R²<br>= 0.9998), y = 3330.4×–1162.3 (R² = 0.9999), and y = 1262.7× + 990.03 (R² = 0.9998) for tenofovir disoproxil<br>fumarate, dolutegravir sodium, and emtricitabine, respectively, and the method was validated as per ICH guidelines.<br>The total run time was 5 min. The limit of quantitation values was found to be 1.9113, 4.8752, and 4.7654 μg/mL and<br>that of the limit of detection values 0.6287, 0.1598, and 0.1568 μg/mL for tenofovir disoproxil fumarate, dolutegravir<br>sodium, and emtricitabine respectively. The proposed RP-UPLC method is simple, precise, and accurate. This method<br>can be used for the regular analysis of pharmaceutical dosage forms. Conclusion: The proposed RP-UPLC method<br>is simple, precise, and accurate. This method can be used for the regular analysis of pharmaceutical dosage forms.</p> ER -