@article{Karpova_2019, title={Development and Validation of the Spectrophotometric Method for the Quantitative Determination of Azlocillin in Pure Substance and Medical Preparation}, volume={12}, url={https://www.asiapharmaceutics.info/index.php/ajp/article/view/2946}, DOI={10.22377/ajp.v12i04.2946}, abstractNote={<p>Objective: The kinetic of conjugated reactions of S-oxidation and perhydrolysis of Azlocillin (Azl) with<br />potassium caroate in alkaline medium is studied by the increase of forming product light absorbance at 275 nm.<br />Materials and Methods: Securopen® - powder Azl sodium in flacons for the preparation of solution for injections<br />(Azl 1.0 g and 5.0 g) was used for analysis. Peroxymonosulfate acid as triple potassium salt 2КНSO5∙КНSO4∙K2SO4<br />(Oxone®) of “extra pure” qualification was used as oxidant. A scheme of peroxyacid oxidation and perhydrolysis<br />conjugated reactions of Azl by means of potassium caroate is proposed. A new spectrophotometric method for<br />the quantitative determination of sodium Azl in Securopen® preparation using potassium caroate (KHSO5) as an<br />analytical reagent was proposed. Results: At pH 2–3 for 1 mole of penicillin, 1 mole of KHSO5 is consumed,<br />and the quantitative interaction is achieved within a time of more than 1 min (observation time). The results were<br />obtained by the recommended procedure for seven replicate titrations of mixtures containing the three species<br />at various concentrations. Relative standard deviation (RSD) = (0.96–2.02) %, δ = (+0.49 … – 0.33)%. It can<br />be seen that Azl can be determined successively with good accuracy and reproducibility. Conclusions: The new<br />procedure was developed and the ability of quantitative determination of penicillin in pharmaceutical preparation<br />Securopen® by spectrophotometric method using potassium caroate (KHSO5) as analytical reagent was shown.</p>}, number={04}, journal={Asian Journal of Pharmaceutics (AJP)}, author={Karpova, Svitlana P.}, year={2019}, month={Feb.} }