@article{Asatryan_2019, title={Tannic Acid-induced Modulation of Poly(ADP-Ribose) Glycohydrolase and NAD+ Content in Rat Liver and Thymocyte Nuclei}, volume={13}, url={https://www.asiapharmaceutics.info/index.php/ajp/article/view/3410}, DOI={10.22377/ajp.v13i04.3410}, abstractNote={<p>Objective: The goal of the present study was to determine whether the treatment of intact rats with<br />tannic acid (TA) could alter activities of the main players in poly(ADP-ribose) (PAR) polymers<br />turnover. Materials and Methods: Animals were treated according to the regulations of the National Center of<br />Bioethics (Armenia). Cell nuclei were isolated according to standard procedure. PAR polymerase 1 (PARP 1)<br />activity was evaluated by NAD+ consumption. PAR glycohydrolase (PARG) protein was estimated by sandwich<br />ELISA method. Data are expressed as mean ± standard deviation (SD). Statistical differences in the results<br />between groups were evaluated by the Student’s t-test. P < 0.05 was considered statistically significant. Results:<br />The results demonstrate relatively stable stimulation of PARP 1 activity in liver and thymocyte nuclei of rats<br />treated with TA. TA stimulated PARP 1 activation in thymocyte and liver nuclei is paralleled with drop in NAD+<br />content for more than 30% below basal level. The concentration of PARG in liver nuclei exceeded the basal<br />value of PARG content in thymocyte nuclei nearly 3.5-fold. In 24 h after administration of TA to animals the<br />content of PARG protein in thymocyte nuclei decreased by 30%. Decrease of PARG content in liver nuclei was<br />more substantial and proceeded for 48 h after the treatment of rats with TA. Conclusion: TA-induced stimulation<br />of PARP 1 in liver and thymocyte nuclei paralleled with decrease in NAD+ and PARG content could alter PAR<br />polymer turnover and mediate genotoxic effect of TA revealed by light microscopy examination of liver.</p>}, number={04}, journal={Asian Journal of Pharmaceutics (AJP)}, author={Asatryan, Anush L.}, year={2019}, month={Nov.} }