@article{Asatryan_2020, title={Effects of Tannic Acid on Cisplatin-Induced Changes in Poly(ADP-Ribose) Polymer Turnover in Rat Liver and Thymocyte Nuclei}, volume={14}, url={https://www.asiapharmaceutics.info/index.php/ajp/article/view/3688}, DOI={10.22377/ajp.v14i03.3688}, abstractNote={<p>Objective: Cisplatin is a powerful antineoplastic drug widely used in the therapy of cancer patients. To attenuate<br />undesirable side effects of the drug, chemotherapeutic regimens are employed based on the use of antioxidant<br />supplementation. The goal of the present study is the examination of the impact of cotreatment with cisplatin<br />and tannic acid (TA) on key members involved in poly(ADP-ribose) polymer (pPARr) turnover in rat liver and<br />thymocyte nuclei, i.e., poly(ADP-ribose)polymerase 1 (PARP 1) activity, poly(ADP-ribose)glycohydrolase<br />(PARG), and NAD+ content. Materials and Methods: Wistar albino rats were randomly distributed in four<br />groups: Control group, treated with cisplatin, tannic acid, and cotreated with tannic acid and cisplatin. The drugs<br />were injected intra-peritoneal. Animals were treated according to regulations of National Centre of Bioethics<br />(Armenia). Cell nuclei were isolated according to standard procedure. PARP 1 activity was evaluated by NAD+<br />consumption. PARG protein was estimated by sandwich Elisa method. Data are expressed as mean ± s.d. Statistical<br />differences in the results between groups were evaluated by the Student’s t-test. A probability (P) value of < 0.05<br />was considered significant. Key Results: Cisplatin and tannic acid displayed hepatotoxic effects in rat liver in 48<br />h after treatment. Although treatment of rats either with cisplatin or TA downregulated NAD+ and PARG content<br />in liver nuclei, the drugs exhibited oppositely directed effects on PARP 1 activity. Cotreatment with cisplatin<br />and TA-stimulated PARP 1 activity in liver nuclei did not affect the basal level of NAD+ and prevented drastic<br />decrease in PARG protein level. Conclusions: Cisplatin-induced inhibition of PARP 1 activity, NAD+, and PARG<br />content in liver nuclei were eliminated after cotreatment of rats with tannic acid.</p>}, number={03}, journal={Asian Journal of Pharmaceutics (AJP)}, author={Asatryan, Anush}, year={2020}, month={Jul.} }