TY - JOUR AU - Dharmamoorthy, G. PY - 2020/06/09 Y2 - 2024/03/28 TI - New Validated Stability-Indicating Reverse-phase High-performance Liquid Chromatography Method for the Simultaneous Estimation of Prazosin and Polythiazide in their Formulations in Human Plasma JF - Asian Journal of Pharmaceutics (AJP) JA - AJP VL - 14 IS - 2 SE - ORIGINAL ARTICLES DO - 10.22377/ajp.v14i2.3621 UR - https://www.asiapharmaceutics.info/index.php/ajp/article/view/3621 SP - AB - <p>Objective: A new simple and precise stability-indicating bioanalytical reverse-phase high-performance liquid<br />chromatography (RP-HPLC) method was developed and validated for the simultaneous estimation of prazosin<br />and polythiazide in their formulation and in human plasma. Materials and Methods: The developed method was<br />successfully used for assaying drug contents in plasma. Isocratic elution mode was carried at Agilent C18 column<br />(150 mm × 4.6 mm, 5 μm particle size) using 0.01 N disodium hydrogen phosphate pH 5.0:acetonitrile (55:45) as<br />mobile phase at flow rate 1.0 ml min at detection wavelength 265 nm. Celecoxib was taken as an internal standard.<br />The method was validated as per ICH guidelines. Results and Discussion: The present validated method can be<br />successfully applied for the estimation of prazosin and polythiazide in human plasma over the concentration range<br />of 12.5–500 ng/m of prazosin and 6.25–250 ng/ml of polythiazide. The method for the determination of prazosin<br />and polythiazide in human plasma using HPLC detection met the acceptance criteria with respect to selectivity,<br />precision, accuracy, linearity, and recovery. Conclusion: The proposed method is simple, rapid, accurate, precise,<br />and appropriate for pharmacokinetic and therapeutic drug monitoring in the clinical laboratories.</p> ER -