TY - JOUR AU - Gaikwad, Asmita V. PY - 2022/01/18 Y2 - 2024/03/29 TI - A High Performance Liquid Chromatography Method Development and Validation for the Estimation of Canagliflozin in Bulk and Marketed Dosage Form JF - Asian Journal of Pharmaceutics (AJP) JA - AJP VL - 15 IS - 04 SE - ORIGINAL ARTICLES DO - 10.22377/ajp.v15i04.4230 UR - https://www.asiapharmaceutics.info/index.php/ajp/article/view/4230 SP - AB - <p>Aim: An efficient and simple high-performance liquid chromatography (HPLC) method has been developed<br />and validated for the determination of canagliflozin in marketed formulations containing canagliflozin.<br />Materials and Methods: The mobile phase used for the chromatographic runs consisted of 5 mMammoniumformate<br />in water: Methanol (25:75, v/v) The separation was achieved on a Symmetry, Waters C 18 (100 mm × 4.6 mm ×<br />3.5 μm) using isocratic mode. Results and Discussion: Drug peaks were well separated and were detected by a<br />UV detector at 290 nm. The method was linear at the concentration range of 1–10 μg/ml for both the formulations.<br />The method has been validated according to the International Conference of Harmonisation guidelines concerning<br />precision, accuracy, and forced degradation. Canagliflozin limit of detection and limit of quantification (LOQ)<br />were 0.0026 ng/ml and 0.008 ng/ml, respectively. Conclusion: The current research epitomizes the report that<br />deals with the development of a stability-indicating HPLC method for the determination of Canagliflozin in two<br />different brands. Canagliflozin is very sensitive so it is unstable in alkaline, oxidative and thermal conditions but<br />stable in UV light or acid conditions.</p> ER -