Multi Component Mode and Derivative Spectrophotometric Methods for the Simultaneous Determination of Timolol maleate and Brimonidine Tartrate

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Mukthinuthalapati Mathrusri Annapurna

Abstract

Introduction: New multicomponent spectrophotometric methods have been established for the simultaneous determination of timolol maleate and brimonidine tartrate in pharmaceutical formulations (eye drops). Timolol is a propanolamine derivative having non-selective beta-adrenergic antagonist action with antihypertensive action. Brimonidine is used to treat glaucoma, or ocular hypertension and timolol can be used as an antihypertensive and anti-glaucoma agent. Materials and Methods: Shimadzu ultraviolet (UV)-1800 model UV-visible spectrophotometer double beam was used for the present study. The three new methods were developed using phosphate buffer pH 7.0. Results and Discussion: The absorption maxima were found at 295 nm for timolol maleate and 247 nm for brimonidine tartrate, respectively. Linearity was observed 1–120 μg/ml for timolol maleate and 1–60 μg/ml for brimonidine tartrate and the three methods were validated as per the ICH guidelines. Conclusions: The three methods can be used for the determination of timolol maleate and brimonidine tartrate in eye drops.

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How to Cite
Annapurna, M. M. (2018). Multi Component Mode and Derivative Spectrophotometric Methods for the Simultaneous Determination of Timolol maleate and Brimonidine Tartrate. Asian Journal of Pharmaceutics (AJP), 12(01). https://doi.org/10.22377/ajp.v12i01.2069
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ORIGINAL ARTICLES