Spectrophotometric determination of nateglinide in bulk and tablet dosage forms

Suresh Jain, Anil Bhandari, Suresh Purohit

Abstract


Nateglinide (NTG) is available as tablet dosage form in 60 mg and 120 mg strength. In the present study, two simple, reproducible and efficient UV spectrophotometric methods for the estimation of this drug in bulk and pharmaceutical
dosage forms have been developed. In method I, methanol-AR was used as solvent, while in method II, Methanol‐AR + 10% V/V 3N NaOH was used as reference solvent. In method I, nateglinide shows λmax at 216 nm, which is then shifted to 225.4 nm on increasing the basicity of the reference solvent in method II.The linearity for nateglinide was observed to be statistically in
the range of 10-100 μg/ml in method I and 100-1000 μg/ml in method II. Both the methods were validated using ANOVA. The recovery studies confirmed the accuracy of the proposed methods.


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References


Available from: http://www.drugs.com /cons/ Nateglinide.html. [last

accessed on 2005 Jun 8].

Available from http://www.rxlist.com /cgi/ generic2/Nateglinide.html.

[last accessed on 2006 Apr].

Available from http://www.pdrhealth.com /drug_info/rxdrugprofiles/

drugs/sta1565.shtml. [last accessed on 2006 Jul].

Yan H, Yang G, Qiao F, Chen Y. Determination of nateglinide in animal

plasma by micellar electrokinetic chromatography and on-line sweeping

technique. J Pharm Biomed Anal 2004;36:169-74.

Bauer S, Stormer E, Kirchheiner J, Michael C, Brockmoller J, Roots I. Rapid and simple method for the analysis of nateglinide in human plasma usin HPLC analysis with UV detection. J Pharm Biomed Anal 2003;31:551-5.

Rajasekaran A, Abdul Hathi MK, Murugesan S, Ajith SN, Devi G Aruna,

Sowmiya G, Dharmar P. Spectrophotometric determination of

nateglinide. Indian J Pharm Sci 2004;66:806-7.

Sagar GV, Rao NV, Sastry BS. Assay of lacidipine in tablets by extraction

spectrophotometry. Indian J Pharm Sci 2004;66:353-5.

ICH Guidance on Analytical Method Validation. In: Proceedings of the

international Conference of Harmonization, Geneva: 1996.

Text on Validation of Analytical Procedures Q2B in; I.C.H. Harmonised

Tripartite Guidelines; Nov. 1996.




DOI: http://dx.doi.org/10.22377/ajp.v3i3.269

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