Development and Validation of the Spectrophotometric Method for the Quantitative Determination of Azlocillin in Pure Substance and Medical Preparation

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Svitlana P. Karpova

Abstract

Objective: The kinetic of conjugated reactions of S-oxidation and perhydrolysis of Azlocillin (Azl) with
potassium caroate in alkaline medium is studied by the increase of forming product light absorbance at 275 nm.
Materials and Methods: Securopen® - powder Azl sodium in flacons for the preparation of solution for injections
(Azl 1.0 g and 5.0 g) was used for analysis. Peroxymonosulfate acid as triple potassium salt 2КÐSO5∙КÐSO4∙K2SO4
(Oxone®) of “extra pure†qualification was used as oxidant. A scheme of peroxyacid oxidation and perhydrolysis
conjugated reactions of Azl by means of potassium caroate is proposed. A new spectrophotometric method for
the quantitative determination of sodium Azl in Securopen® preparation using potassium caroate (KHSO5) as an
analytical reagent was proposed. Results: At pH 2–3 for 1 mole of penicillin, 1 mole of KHSO5 is consumed,
and the quantitative interaction is achieved within a time of more than 1 min (observation time). The results were
obtained by the recommended procedure for seven replicate titrations of mixtures containing the three species
at various concentrations. Relative standard deviation (RSD) = (0.96–2.02) %, δ = (+0.49 … – 0.33)%. It can
be seen that Azl can be determined successively with good accuracy and reproducibility. Conclusions: The new
procedure was developed and the ability of quantitative determination of penicillin in pharmaceutical preparation
Securopen® by spectrophotometric method using potassium caroate (KHSO5) as analytical reagent was shown.

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How to Cite
Karpova, S. P. (2019). Development and Validation of the Spectrophotometric Method for the Quantitative Determination of Azlocillin in Pure Substance and Medical Preparation. Asian Journal of Pharmaceutics (AJP), 12(04). https://doi.org/10.22377/ajp.v12i04.2946
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ORIGINAL ARTICLES