Incompatibility studies by high performance thin‐layer chromatography: In case of curcumin

Alankar Shrivastava, Jitendra Sharma, Saurabh Jain, Kanhaiya Lal Aggrawal


Curcumin[1,7‐bis (4‐hydroxy‐3‐methoxy‐phenyl) hepta‐1, 6‐diene‐3, 5‐dione] is one of the component present in the turmeric. Curcumin has been in use for its medicinal benefits since centuries and its therapeutic potential is
continuously explored through various researchers throughout the world. To investigate the interaction of curcumin with commonly used excipients such as microcrystalline cellulose, starch, colloidal silica, talc, ascorbic acid, lactose, ethyl
cellulose (EC), sodium carboxymethylcellulose (Na‐CMC), hydroxyl propyl methyl cellulose and magnesium stearate. High performance thin‐layer chromatography (HPTLC) is commonly used technique for the determination of phytoconstituents,
but its application in incompatibility studies is still not investigated. Thus, we initiated our study with HPTLC followed by Fourier transform infrared and differential scanning calorimetry. Since interaction of curcumin with ascorbic acid, EC, Na‐CMC and Mg‐stearate confirmed by all three techniques these four excipients should be avoided during the formulation development of curcumin. The presented study also establishes HPTLC’s usefulness in such interaction studies.

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