Nanoemulsion formation and characterization by spontaneous emulsification: Investigation of its antibacterial effects on Listeria monocytogenes
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Atransparent oilâ€inâ€water nanoemulsion system consisting of eucalyptus oil, Tweenâ€20 as organic phase and water as an
aqueous phase was developed using a low energy emulsification method. Physicochemical properties such as dropletÂ size, optical transparency, and longâ€term stability was studied. The stable eucalyptus oil nanoemulsion (1:2) having meanÂ droplet size in the range of 50-100 nm with a polydispersity index <0.2. The optimized nanoemulsion formulation exhibitedÂ significantly higher antibacterial activity by the well diffusion method against Listeria monocytogenes. Further, alteration inÂ the membrane integrity was assessed, and it is found higher for nanoemulsion treated cells than control cells. Atomic forceÂ microscopic observations showed distorted morphology of treated bacterial cells. These results propose the possible use ofÂ eucalyptus oil nanoemulsion in the food industries.
Key words: Antibacterial, eucalyptus oil, Listeria monocytogenes, nanoemulsion, spontaneous emulsification
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