Anticancer Potential of Tabernaemontana divaricata Extracts against HeLa and Vero Cell Lines

Abstract

Background: The history of India has recorded about its ancient medical practices, which date back to centuries when
the use of green plants of various kinds was used to treat most of the diseases in such critical periods. Such one is the
perennial evergreen shrub Tabernaemontana divaricata, which has been used as traditional medicine. It is mainly
famous with its phenols and flavonoids. These compounds are associated with different pharmacological activities.
Objective: Therefore, the leaves of T. divaricata were collected, extracted, and studied in this research to establish
their anticancer effects. Nevertheless, its anticancer properties have received less research; hence, the objective of the
research was to examine the in vitro anticancer effects of T. divaricata leaf extract isolated with the aid of a crude
solvent extraction. Materials and Methods: Past research has demonstrated that it has possible anti-inflammatory,
anti-microbial, and antioxidant properties. The anticancer activity was confirmed by 3-(4,5-dimethylthiazol-2-yl)-2,5-
diphenyltetrazolium bromide assay used to evaluate the inhibition of cell growth in the in vitro anticancer research
that was done on Vero and HeLa cell lines. Results: The findings revealed the anti-cancer activity of T. divaricata on
the HeLa cell lines. In addition, docking showed that 2,2-dibromocholestanone has high binding affinity to Caspase
3, p53, and nuclear factor kappa -B and indicates potential use in anticancer. Conclusion: These results indicate the
potential anticancer properties of T. divaricata leaf extract and support its possible use in anticancer research.