Formulation and in-vitro evaluation of Chitosan films containing tetracycline for the treatment of periodontitis

Mohammed Gulzar Ahmed, R Narayana Charyulu, N M Harish, Prabhakar Prabhu


Tetracycline is a broad spectrum antimicrobial agent, which is formulated into films and evaluated for the treatment of periodontitis. Chitosan films containing tetracycline in three different concentrations (10, 20, and 30% w/w to the
weight of polymer) were prepared by the solution casting method, using 1% v/v acetic acid solution. The prepared films were evaluated for various properties such as weight variation, tensile strength, stability studies, in-vitro release, and mass
balance studies. Further films were cross-linked in order to extend the drug release. Average weight and thickness among the different films was uniform.Tensile strength was maximum for plain films and minimum for films containing the highest percentage of the drug or cross-linked films. The stability studies did not show any significant changes. Static dissolution studies showed a burst release initially followed by a progressive fall in the release of the drug, and also showed extended release when cross-linking was attempted. The in-vitro release kinetics of tetracycline followed the zero order pattern. The
fact that the mass balance studies done after in-vitro dissolution did not deviate by more than 3% from the experimental drug content, confirms that the drug is in free form rather than bound to the polymer.

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Brown LJ, Brunella J, Kingman A. Periodontal status in the United States,

-1991: Prevalence, extent, and demographic variation. J Dent Res

; 75: 672-83.

Yeung FI, Newman HN, Addy M. Subgingival metronidazole in acrylic

vs. chlorhexidine irrigation in the control of chronic periodontitis.

J Periodontol 1983;54:651-7.

Kornman KS. Controlled release local delivery antimicrobials in

periodontics: Prospects of the future. J Periodontol 1993; 64:782-91.

Slots J, Rams TE. Antibiotics in periodontal therapy: Advantages and

disadvantages. J Clin Periodontol 1990; 17:479-93.

Peh KK, Wong CF. Polymeric films as a vehicle for buccal delivery: Swelling, mechanical and bioadhesive properties. J Pharm Sci 1999;2:53-61.

Pandit JK. Targeted devices for periodontal disease. In: Controlled and

novel drug delivery. NK Jain editor. chapter 6. Vol 2. CBS Publishers and

distributors; 2004. p. 130-46.

Kim TS, Klimpel H, Fiehn W, Eickholz P. Comparison of the

pharmacokinetic profile of two locally administered doxycycline gels

in crevicular fluid and saliva. J Clin Periodontol 2004;31:286-92.

Ravi Kumar MN. A review of chitin and Chitosan applications. React

Funct Poly 2002; 46:1-27.

Ilango R, Jayakar B, Kavimani S. Chitosan as a new pharmaceutical

excepient. Eastern Pharmacist 1998; 483:47-8.



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