Comparison of the disc diffusion assay with spectrophotometer technique for antibacterial activity of diclofenac sodium, indomethacin and mefenamic acid

Main Article Content

Ali Abdul Hussein S AL-Janabi


Non-steroidal anti-inflammatory drugs (NSAIDs) contain a wide range of effective drugs that use in treatment of inflammatory diseases. Chemical agents of non-steroidal anti-inflammatory drugs including diclofenac sodium, indomethacin and mefenamic acid, were screened for antibacterial activity by using two methods: Disc diffusion assay and spectrophotometer technique. From the results of disc diffusion method, Staphylococcus aureus exhibited susceptibility to diclofenac sodium and mefenamic acid. While, spectrophotometer method showed that Paracoocus yeei was susceptible to all tested compounds. Diclofenac sodium had loweress MIC (2.5 mg/ml) to inhibit all of seven isolated strains. The application of spectrophotometer technique in this study considers more suitable method than those of disc diffusion test to reveal antibacterial activities of tested agents, especially with indomethacin.


Download data is not yet available.

Article Details

How to Cite
S AL-Janabi, A. A. H. (2014). Comparison of the disc diffusion assay with spectrophotometer technique for antibacterial activity of diclofenac sodium, indomethacin and mefenamic acid. Asian Journal of Pharmaceutics (AJP), 3(2).


Paje ML, Kuhlicke U, Winkler M, Neu TR. Inhibition of lotic biofilms by

diclofenac. App Microbiol Biotechnol 2002;59:488-92.

Parfitt K. Martindale, the complete drug reference. 32nd ed. PhP. U.S.A:

Dutta NK, Mazumdar K, Baek MW, Kim DJ. in vitro efficacy of diclofenac

against Listeria monocytogenes. Eur J Clin Microbiol Infect Dis


Mazumdar K, Dutta NK, Dastidar SG, Motohashi N, Shirataki Y.

Diclofenac in the management of E. coli urinary tract infections. In vivo


Dutta NK, Dastidar SG, Kumar A, Mazumdar K, Ray R, Chakrabarty AN.

Antimycobacterial activity of the anti-inflammatory agent diclofenac

sodium, and its synergism with streptomycin. Braz J Microbiol


Zhang Y, Zhang H, Sun Z. Susceptibilty of Mycobacterium tuberculosis

to weak acids. J Antimicrob Chemother 2003;52:56-60.

Dalby AB, Frank DN, St. Amand AL, Bendele AM, Pace NR. Culture-

Independent analysis of indomethacin-induced alterations in the rat

gastrointestinal microbiota. Appl Environ Microbiol 2006;72:6707-15.

Linder H, Engberg I, van Kooten C, de Man P, Svanborg-Eden C. Effects

of anti-inflammatory agents on mucosal inflammation induced by

infection with gram-negative bacteria. Infect Immun 1990;58:2056-60.

Van der Flier M, Geelen SP, Kimpen JL. Anti-inflammatory adjuvant

agents in bacterial meningitis. Curr Med Chem 2002;1:55-62.

National Committee for Clinical Laboratory Standards. Performance

Standards for Antimicrobial Disk Sususceptibility Test: Approved

Standard M2-A6.NCCLS.Wayne, PA: 1997.

Domínguez MC, de la Rosa M, Borobio MV. Application of a

spectrophotometric method for the determination of post-antibiotic

effect and comparison with viable counts in agar. J Antimicrob

Chemother 2001;47:391-8.

NCCLS. Methods for dilution antimicrobial susceptibility testing for

bacteria that grow aerobically; approved standard-sixth edition. NCCLS

document M7-A6. NCCLS, Wayne. Pennsylvania: 2003.

Kruszewska H, Zareba T, Tyski S. Estimation of antimicrobial activity

of selected non-antibiotic products. Acta Pol Pharm 2006;63:457-60.

Fuchs PC, Barry AL, Brown SD. Comparative in vitro antimicrobial

activity of a new carbapenem, E1010, and tentative disc diffusion test

interpretative criteria. J Antimicrob Chemother 2001;48:23-8.

Derle DV, Bele M, Kasliwal N. in vitro and in vivo evaluation of mefenamic acid and its complexes with β-cyclodexin and HP-β-cyclodexin. Asian Journal of Pharmaceutics 2008;21:30-35.

Kowalczyk E, Kopff A, Blaszczyk J, Fijalkowski P, Kopff M. The influence

of selected nonsteroidal anti-inflammatory drugs on antioxidative

enzymes activity. Pol Arch Med Wewn 2006:115:112-7.